Clontech pabai
WebJan 24, 2024 · The Y1H Gold strain is the compatible strain for pABAi vector provided by Clontech. Can we use Ym4271 (the strain for Y1H assay and has the same genotype of Y1H Gold strain) instead of Y1H Gold ... WebUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix …
Clontech pabai
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WebVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. http://www.biofeng.com/zaiti/jiaomu/pAbAi.html
WebpAbAi. Yeast integrating vector for cloning a DNA bait sequence to be used in a one-hybrid screen. Sequence Author: Clontech (TaKaRa) WebMar 3, 2024 · The RT300 sequence of QH34 was amplified and cloned into the pAbAi vector to form the pBait-AbAi plasmid, Y1H-RT300. The Y1H-RT300 plasmid was integrated into the genome of yeast ... (Clontech). For high stringency in the interaction tests to the RT300 sequence, ...
WebJan 17, 2024 · The resulting pAbAi-bait plasmid was transformed into a Y1HGold strain to generate a bait reporter strain. The full-length CDSs of the HbMYC2/3/4 genes were then amplified with gene-specific ... WebNov 4, 2024 · The linearized PeHA1-pro-pAbAi plasmid was transferred into Y1H yeast, and the positive strain was identified by testing the AbA inhibition. The PeHA1-pro-pAbAi strain was plated on synthetic/–uracil (SD/-Ura) medium supplemented with 0, 700, and 800 ng ml −1 AbA. The Y1H strain was completely inhibited at 800 ng ml −1 AbA.
WebJul 5, 2024 · Subsequently, we inserted the amplification product into the pMD19-T vector, and subcloned into the pAbAi vector upstream of the AbAr reporter gene ... (Clontech, PT1172-1). The yeast transformants were selected on SD/-Trp and transactivation activity was evaluated on SD/-Trp-His-Ura-Ade/ + X-α-gal plates. The yeast cells on the …
WebOct 19, 2024 · Saccharomyces Y1H Gold (Clontech, Palo Alto, CA, USA) was used as the host. We first digested the pAbAI- proGRP23 vector using Bst BI), and then integrated it into Y1H Gold. The bait strain (Y1H-pAbAI- proGRP23 ) was screened for self-activated on synthetically defined (SD)/−Ura with aureobasidin A (AbA*). cut top off wine bottleWebMar 9, 2024 · Four tandem copies of the GCC-box and mGCC-box mutants were cloned into the pAbAi vector and used as bait. The yeast transformants were incubated for 3 … cheap electric ranges with free deliveryWebYeast One Hybrid - North Carolina State University cut top off bottleWebApr 16, 2024 · Background This study aimed to identify the transcription factors of nitrate reductase genes (NIA1 and NIA2) promoters and hypothetical cis-element of NRE2. Based on the constructed cDNA library of Nicotiana tabacum K326, a yeast one-hybrid system was established using the Matchmaker® Gold Yeast One-Hybrid Library Screening System … cheap electric longboardWebAug 25, 2024 · The NAC (NAM, ATAF1/2, and CUC2) transcription factors are one of the largest families of transcription factors in plants and play an important role in plant development and the response to adversity. In this study, we cloned a new NAC gene, SlNAC10, from the halophyte Suaeda liaotungensis K. The gene has a total length of … cut top of hair with clippershttp://www.labbase.net/Supply/SupplyItems-4229347.html cheap electric rates for businessesWebDec 18, 2005 · Transient expression of heme-thiolate P450. Initial experiments assessed the level of enzyme activity from transiently expressed Md-C4H1 and the effect of Ad-P450 reductase 10 days after Agrobacterium infiltration. The extent of conversion from cinnamic acid to p-coumaric acid, measured by HPLC, was similar for both the MdC4H1 and the … cheap electric mountain bikes