Phospho-beclin-1

WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold WebSpecificity / Sensitivity. Phospho-Beclin-1 (Ser90) Antibody recognizes endogenous levels of Beclin-1 protein only when phosphorylated at Ser90. The antibody may not react with …

Phospho-Beclin 1 (Ser90, Ser93, Ser96) Polyclonal Antibody

WebThe effects on the PI3K/Akt/mTOR axis (PI3K, Akt, phospho-Akt, mTOR, and phospho-mTOR) during FeHV-1 infection were examined in Western blot analysis. We found an increase in … WebBeclin-1 was purified and immunoblotted as in Fig.3b. (d) Wild-type or ULK def MEF were incubated with or without amino acids. Beclin-1 was purified and immunoblotted as in Fig.3a. Two unique experiments were performed. Article Snippet: Anti-phospho-Beclin-1 (S14) was obtained from Abbiotec, San Diego, CA (Cat#254515). bird\u0027s eye view optometry league city https://gameon-sports.com

Phospho-Beclin 1 (Ser15) Polyclonal Antibody (PA5 …

WebObjective: Hydrogen sulfide (H 2 S), a gaseous signal molecule, plays a crucial role in many pathophysiologic processes in the cardiovascular system. Autophagy has been shown to participate in the occurrence of many cardiac diseases. Increasing evidences indicated that H 2 S regulates myocardial structure and function in association with the altered … WebPhospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb recognizes endogenous levels of Beclin-1 protein only when phosphorylated at Ser93. This antibody recognizes single phosphorylation of Ser93 to a greater extent than dual phosphorylation at Ser93 and Ser96. This antibody may also weakly detect an unidentified band at approximately 70 kDa. WebA phosphotyrosine antibody was used for phospho-BECLIN-1 and phospho-BCR-ABL detection. (B) Immunoprecipitation of BCR-ABL with BECLIN-1 and (C) BECLIN-1 with ABL in K562 cells confirms the interaction reciprocally and at endogenous level. (D) GST-pull-down of BCR-ABL with recombinant GST-Beclin-1 in K562 cells corroborated the interaction ... dance of luck buddy valorant

Phospho-Beclin 1 (S234) ELISA Kit (ab279747) Abcam

Category:Rabbit Anti-Human Phospho-beclin 1(S64) RayBiotech

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Phospho-beclin-1

CaMKII-mediated Beclin 1 phosphorylation regulates …

WebSep 12, 2013 · Lysates were immunoprecipitated with anti-Beclin 1 followed by western blot analysis with anti-phospho-Beclin 1 Y233. (F) Western blot detection of phospho-Beclin 1 Y233 in NSCLC cell lines with or without active EGFR mutations. Phospho-Beclin 1 Y233 was detected as in (E). See text for information on cell lines. WebPhospho-Beclin 1 (S234) ELISA Kit (ab279747) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in …

Phospho-beclin-1

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WebNov 11, 2024 · The co-localization of 14-3-3ζ and beclin 1 was detectable in primary HCC tissues. To simulate in vivo tumour microenvironment (hypoxia), CSQT-2 and HCC-LM3 cells were exposed to 2% oxygen for 24 hours. The protein levels of 14-3-3ζ and phospho-beclin 1 S295 peaked at 12 hours following hypoxia. WebNov 7, 2015 · Westernblotting results failed showany phospho-Raptor phospho-p53bandsinbothcontrol andtreatedcells (datanot shown). control,we checked phosphorylationlevels starvedcells phospho-AMPK phospho-ACCoccurred after 6–18 autophagyinduction (Western blot- ting LC3I/II) were observed (Fig. Wealso measured …

WebKey features and details Rabbit polyclonal to Beclin 1 (phospho S295) Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Protein Recombinant … Web在本研究中，ROT 通过增加 DEPTOR 水平和 p -/t-AMPK 比率。此外，ROT 通过降低 p-/t-ERK1/2 比率和 beclin-1 水平来增加 p-/t-Akt 比率和糖原合酶激酶-3β (GSK3β) 活性。ROT 还通过在 Triton X-100 可溶性和不溶性细胞裂解物部分中诱导自噬体形成来促进 LC3B-I 脂化为 …

WebSpecificity / Sensitivity. Phospho-Beclin-1 (Ser93/96) Antibody recognizes overexpressed levels of Beclin-1 protein only when phosphorylated at Ser93 and Ser96 (Ser91 and Ser94 … WebMay 26, 2024 · Beclin 1 protects cells from necroptosis in an autophagy-independent manner. Beclin 1 is a modulator of the apoptosis pathway [].To further identify whether Beclin 1 is involved in TNFα-induced ...

WebWestern blot - Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252) Western blot analysis of extracts of various cell lines, using Phospho-Beclin 1-Ser15 antibody (AP1252) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L ...

WebThe NeuroMab™ anti-Phospho-Beclin-1 (Ser15) antibody recognizes transfected levels of Beclin-1 protein only when phosphorylated at Ser15 (which corresponds to Ser14 in … bird\u0027s eye view of pathfinder landing siteWebMar 3, 2015 · 1. Introduction. Glioblastoma (GB) is the most frequent primary brain neoplasm and one of the most lethal tumors. Standard treatment is the maximal safe … bird\u0027s eye view spring clean upWebPolyclonal Antibody for studying Beclin 1 (Ser90) phosphate. Cited in 2 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Beclin-1 (Ser90) Antibody (CST #86455) is ready to ship. dance of lanterns bannerWebOne of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast … dance of life artWebInvitrogen Anti-Phospho-Beclin 1 (Ser15) Polyclonal, Catalog # PA5-104576. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC (P)) applications. This … dance of luck gun buddyWebWestern Blot analysis of Phospho-Beclin 1 (Ser90) in various cell lines using a Phospho-Beclin 1 (Ser90) Polyclonal antibody ( Product # PA5-121359) at a dilution of 1:500. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary ... bird\u0027s eye view photography fruitWebThe Beclin-1 S14 phosphorylation by ULK is required for full autophagic induction in mammals and this requirement is conserved in C. elegans. Our study reveals a molecular link from ULK1 to activation of the autophagy specific VPS34 … dance of little swans